Under the condition, the average number of NOTA chelators per pertuzumab molecule was determined to be 1.9 by the isotope dilution method (Fig. The conjugation of p-SCN-Bn-NOTA (2-S-(4-Isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid) to pertuzumab (20:1 molar ratio) was performed at pH 8.5 for 3 h at room temperature, followed by purification by fast protein liquid chromatography (FPLC). Pertuzumab conjugation, 67Cu radiolabeling, and in vitro stability A HER2 targeting antibody, pertuzumab, was used as a model antibody to construct the desired radioimmunotheranostic conjugate. In this work, we evaluated the use of 67Cu produced by the electron accelerator method with the aim to reassess the role of 67Cu in radioimmunotherapy coupled with the up-to-date SPECT technology.
ZENON 7.0 LBL STATE TRIAL
Indeed, a clinical trial with 67Cu-Octreotate was reported in 2019 30, and a few preclinical theranostic studies with 67Cu have been seen recently 31, 32, 33.
Due to this breakthrough in 67Cu production, no-carrier-added 67Cu has been made available in large scales at the National Isotope Development Center 29. The specific activity of 67Cu so produced has reached over 5.55 GBq/μg (150 mCi/μg) 26, which is a big leap as compared to that (0.69 GBq/μg or 18.6 mCi/μg) obtainable by conventional 68Zn(p, 2p) 67Cu reaction 28. A major breakthrough was reported in the 68Zn(γ,p) 67Cu reaction by irradiating a highly enriched 68Zn (98.97%) target with high energy γ rays produced by bremsstrahlung conversion of electrons from electron accelerator 26, 27. However, therapeutic quantities of 67Cu at high specific activity were not achieved until recently 21. Of note, the branching ratios of 67Cu’s γ-emissions are much higher than those of 177Lu’s, which would provide the much-needed imaging sensitivity.ĭespite the potential for both imaging and therapy, the use of 67Cu for targeted radionuclide therapy has been hampered for decades by its limited supply and low specific activity. Surprisingly to some extent, in the reported nuclear medicine applications of 67Cu, its γ-emissions at 93 keV (16%) and 185 keV (49%) 14 are largely neglected for SPECT imaging. More importantly, PET imaging can be made readily available by simply swapping 67Cu with 64Cu in the same radiopharmaceutical when desired for pre-screen of patients for targeted 67Cu-radionulide therapy or dosimetry estimation. Given that copper radioisotopes are chemically identical, the same bifunctional chelators that have been developed for 64Cu radiopharmaceuticals can be used directly for 67Cu radiopharmaceuticals 13. Of them, 64Cu has been widely used for preclinical and clinical PET studies due to its moderate half-life (t 1/2 = 12.7 h), low positron energy, and availability 13. In addition to 67Cu, copper has several other radioisotopes ( 60Cu, 61Cu, 62Cu, and 64Cu) that have been reported for the development of PET radiopharmaceuticals. The β − emissions from 67Cu permit the irradiation of several layers of tumor cells and its shorter half-life (t 1/2 = 2.58 d) is more optimal for patient care.
ZENON 7.0 LBL STATE CODE
IATA airport code is LBL.Copper-67 (t 1/2 = 2.58 days) decays by β − ( \(\text\): 562 keV], which is slightly higher than that of 177Lu, 67Cu has long been suggested for radiotherapy since 1980s 11, 12. Liberal Mid-America Regional Airport is located approximately 2.2 miles / 3.5 kilometers west of Liberal and about 64.3 miles / 103.5 kilometers south of Garden City. Liberal Mid-America Regional Airport (LBL)ĭistance from Liberal Mid-America Regional Airport to Alva is 127.9 miles / 205.8 kilometers. Will Rogers World Airport is located approximately 7.0 miles / 11.3 kilometers southwest of Oklahoma City and about 7.0 miles / 11.3 kilometers east of Mustang. Will Rogers World Airport (OKC)ĭistance from Will Rogers World Airport to Alva is 114.4 miles / 184.1 kilometers. Dodge City Regional Airport is located approximately 2.9 miles / 4.7 kilometers east of Dodge City and about 51.6 miles / 83.0 kilometers east of Garden City. Dodge City Regional Airport (DDC)ĭistance from Dodge City Regional Airport to Alva is 97.5 miles / 156.9 kilometers. Eisenhower National Airport is located approximately 6.0 miles / 9.6 kilometers southwest of Wichita and about 11.5 miles / 18.6 kilometers northwest of Derby.
Eisenhower National Airport (89.6 miles / 144.3 kilometers)
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